Journal: Nature genetics

Article Title: MicroRNA Mirn140 modulates Pdgf signaling during palatogenesis.

doi: 10.1038/ng.82

Figure Lengend Snippet: Figure 8 Model of how Mirn140 modulates Pdgf signaling during palatogenesis. (a) Pdgfra signaling is required for neural crest cell dispersion. Neural crest cells (yellow) express pdgfra (red) and mirn140 (blue) as they disperse into regions of pdgfaa expression (plus signs). Mirn140 inhibits Pdgfra production (pink arrow), but the overall level of Pdgf signaling is sufficient to promote crest dispersion along Pdgfaa-positive pathways. (b) When rostrally migrating crest cells reach the optic stalk (os), stoichiometric differences in pdgfra and Mirn140 levels regulate their final migration. To envelop the optic stalk (green), crest cells require higher levels of Pdgfra. Crest cells that migrate on toward the oral ectoderm (oe, blue) must first decrease the levels of Pdgfra using Mirn140 in order to leave the optic stalk. Rc, rostral crest; cc, caudal crest.

Article Snippet: Affi-gel Blue gel (Bio-Rad) was incubated overnight at 4 1C with 10 mg/ml rat recombinant PDGFAA (R&D Systems) or BSA (Sigma).

Techniques: Dispersion, Expressing, Migration